| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.040094 |
| Chromosome: | chromosome 4 |
| Location: | 1710976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212250 | (1 of 82) IPR020846 - Major facilitator superfamily domain; GT77 family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCAGCGGCCCGTGGTCCCGGTACGAGTCCCTGCGGCCGCCGCGTAGGA |
| Internal bar code: | ACGATTTAGCGGTTGTGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1300 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTCCCTGAGTCCCTGAG |
| Suggested primer 2: | GGGGATAGCCATTCATGCGA |