| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040112 |
| Chromosome: | chromosome 2 |
| Location: | 5277685 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g112701 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCATGTCACTGGTGCCCCATGTGACACCAGGGCTTTACCAACGTAACC |
| Internal bar code: | GGTAATGCGACTGCTTCTTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3456 |
| LEAP-Seq percent confirming: | 96.875 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAACGTACTGTTTGGCG |
| Suggested primer 2: | TGTTCCTGCCATGCCTACAG |