| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040126 |
| Chromosome: | chromosome 7 |
| Location: | 4176287 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342250 | PFD2 | Prefoldin molecular chaperone, subunit 2; (1 of 1) K09549 - prefoldin subunit 2 (PFDN2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACTTCATGCAGCACCAACTGGTGATGGACGCGCTCCAGAAGCTGGAT |
| Internal bar code: | GTGTTGTGGGCGGCACGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1879 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCCGCCTACGACTTGGAG |
| Suggested primer 2: | GAAGGCCAGGAAGATGTGCT |