| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040130 |
| Chromosome: | chromosome 17 |
| Location: | 1499323 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706850 | (1 of 1) PF08551 - Eukaryotic integral membrane protein (DUF1751) (DUF1751) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCTCGCTGTTCGAATGACCTCTGTATGCCGCTGCGCTCTCGTGGCG |
| Internal bar code: | GATTTGATTGCGCGATGATCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1882 |
| LEAP-Seq percent confirming: | 98.4615 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTACAGACCTTCGCTGGC |
| Suggested primer 2: | ATTCAAGGTGGGAGCTGTGG |