Insertion junction: CLIP2.040138_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.040138
Chromosome:chromosome 16
Location:4465874
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g667900 (1 of 11) IPR001471//IPR016177//IPR031112 - AP2/ERF domain // DNA-binding domain // AP2-like ethylene-responsive transcription factor; ANT-subfamily ortholog CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACGCGTCGCCCTCGCTGCCACCAGCACCGCCGGCACTGCCGCCGCCGCC
Internal bar code:ATGGTCTCTCGGTCCGTTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGCTGGTTGTGACTGTCA
Suggested primer 2:CCGCCAACCAGCATTGAATC