Insertion junction: CLIP2.040170_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.040170
Chromosome:chromosome 11
Location:2473882
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095113 DIV24,TFCE1,TFC-E Tubulin-folding cofactor subunit; (1 of 1) IPR000938//IPR029071 - CAP Gly-rich domain // Ubiquitin-related domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAACATCCGTAAACCGTCGCTTTGCTGCATCGCATACACTGTCTTTGCG
Internal bar code:AGTACATACAGGAACCATCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:4580
LEAP-Seq percent confirming:95.6044
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:91

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAACCCCTTCTGTCTCGC
Suggested primer 2:GCTTGCTTTGCGTTCTGGAA