| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040196 |
| Chromosome: | chromosome 5 |
| Location: | 3058594 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800572 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGTAAAATGGTGACTTCTGAGGCCAGGGATGTGGAAAGAGTGAGGGA |
| Internal bar code: | TCGGTTCGACACTTTGGGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACAAGGTCATCCGCTCC |
| Suggested primer 2: | CGGACGATCTGGAGAACGAG |