| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040197 |
| Chromosome: | chromosome 10 |
| Location: | 5215275 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455950 | FAP407 | Flagellar Associated Protein 407; (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGGTCAGCGTGCTGCCTCAGTGTTGGTGTGCGCGCGACTGATTCAGT |
| Internal bar code: | GGGGTACAAAGGCTCACCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3564 |
| LEAP-Seq percent confirming: | 99.0476 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGATGGGGCCAGGTAGT |
| Suggested primer 2: | CGTTGAGCTTTTTCGTGCCA |