Insertion junction: CLIP2.040201_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040201
Chromosome:chromosome 7
Location:5385631
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g350400 PIN3 Peptidyl-prolyl cis-trans isomerase, parvulin-type; (1 of 1) 2.7.7.80//2.8.1.11//5.2.1.8 - Molybdopterin-synthase adenylyltransferase // Molybdopterin-synthase sulfurtransferase / Molybdopterin synthase sulfurylase // Peptidylprolyl isomerase / Rotamase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAAACACGTGGACGTACAGCTTGTGGACGTGCGTGAGGACTTCGAGTT
Internal bar code:GCTCTATGGTAGGGTATGTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1855
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGTGTGTTGTGATTGCTG
Suggested primer 2:GCTGCACGCTTCGTATCTTG