| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.040212 |
| Chromosome: | chromosome 2 |
| Location: | 4953003 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110300 | DNJ25 | (1 of 62) PF00226 - DnaJ domain (DnaJ); DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTGCCATGGCTGTCGACCGGGCACTTCGGTGTGGACCACAGGGCGAC |
| Internal bar code: | TTTAGGTTGTGTACAGCATCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2473 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAACAATCACAACGCCT |
| Suggested primer 2: | ATAGTATGGCCGTCAGCACG |