Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.040225 |
Chromosome: | chromosome 14 |
Location: | 2781473 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g626466 | (1 of 30) PTHR24349:SF79 - NITROGEN NETWORK KINASE 1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGCGCCCCAGACCCCCCCCTCGCTCTATTGCAATAGTTGTTTGGTTTG |
Internal bar code: | TGATGGGGATGGAGACGGGCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 322 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 6 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCACACACACACACACAAT |
Suggested primer 2: | TGTAGTGGCGGTAGGGCTAT |