Insertion junction: CLIP2.040237_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040237
Chromosome:chromosome 10
Location:3196418
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g441900 FAP145 (1 of 1) PF15739 - Translin-associated factor X-interacting N-terminus (TSNAXIP1_N); Flagellar Associated Protein 145 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCCACACCGCGCCACGCACCATGCGCTCGTCCACCACCGCCAGCTCCG
Internal bar code:TAGGCAGACACAATTCTCGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3121
LEAP-Seq percent confirming:98.8095
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:CATCGCCCTTACCCTCGTAC
Suggested primer 2:CCACTTCCCGCTTGAGTGAT