| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.040238 |
| Chromosome: | chromosome 1 |
| Location: | 8067660 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g062902 | (1 of 1) K15148 - mediator of RNA polymerase II transcription subunit 7 (MED7) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGCCGTGCCATGAATCTCTGTTTGTAGGGTGCTTGTGTGCGCCCCC |
| Internal bar code: | ACTTTACGGGCCGCGGGACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 209 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCTCACCAACATGCTA |
| Suggested primer 2: | AATGCACCGAAGACTGCAGA |