Insertion junction: CLIP2.040250_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040250
Chromosome:chromosome 12
Location:7116414
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g559750 PRL2 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGGGCAGTAGCATAACGCGCCCATCAACACAGTCTTGCGCTGCGCCTG
Internal bar code:GAATATTGCGGTCTACTGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:509
LEAP-Seq percent confirming:69.2308
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GAACATGGGACGAGGAGTGG
Suggested primer 2:CGACCAACCGACAAACCAAC