Insertion junction: CLIP2.040263_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.040263
Chromosome:chromosome 2
Location:2708235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g093550 CYG44 (1 of 1) IPR000014//IPR001054//IPR029787 - PAS domain // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGTCGGTGTCGCCCTCTCCCCTGGACGGCCGCACGGCGCGGCAACTGG
Internal bar code:AGGGATGAGTCGGCTGGCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3304
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATAGCTGTCAAACGTCG
Suggested primer 2:CTGACAACTCGCTTGGGTCT