Insertion junction: CLIP2.040312_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040312
Chromosome:chromosome 1
Location:4082718
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g027100 IMP2 Mitochondrial inner membrane signal peptidase; (1 of 1) K09648 - mitochondrial inner membrane protease subunit 2 (IMP2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGCGTGGGCGCCATCGAGCTGCCCTCCACCGGCAGTACGCTGACGACC
Internal bar code:CTGATAACAGTGAAGCGTCACG

Confirmation - LEAP-Seq

LEAP-Seq distance:2585
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAGTGTGTTGCAGGCTTG
Suggested primer 2:GGCGATCGTTTGGCTGTTAC