| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040314 |
| Chromosome: | chromosome 16 |
| Location: | 2624915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661300 | THB10 | (1 of 6) PTHR10681//PTHR10681:SF118 - THIOREDOXIN PEROXIDASE // SUBFAMILY NOT NAMED; Truncated hemoglobin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATGATCAAGCAGCAGGGCTTCAGCCCCGAGGACTTCGAGGTGGGCATG |
| Internal bar code: | GCTTCGGTGGAGTTTTATTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3413 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAACTTGCAAGAGCCC |
| Suggested primer 2: | TTTACCAGGGCGGGATTGAC |