| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040317 |
| Chromosome: | chromosome 6 |
| Location: | 5491742 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286350 | UMM6 | UbiE/COQ5 methyltransferase family protein; (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGGTGGCGACGCTGCGGCGCCGCGTGCGCGGTCAGCCCGAGCCGCTG |
| Internal bar code: | GCGGATTAATTGTACAACTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5521 |
| LEAP-Seq percent confirming: | 98.125 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 160 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTCGAGCAGTTTCAGGT |
| Suggested primer 2: | CGGGCTTTCGTTTCGGTTTT |