| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.040349 |
| Chromosome: | chromosome 3 |
| Location: | 2481113 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g159200 | UBC8 | (1 of 2) K10589 - ubiquitin-protein ligase E3 C (UBE3C); Putative ubiquitin-protein ligase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAACTCCAGCAGTGCCAGGCCGCCCGGGATGCGCTCCGCCGCGGGGTT |
| Internal bar code: | GTATTAGATCTAGTCGCAGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2677 |
| LEAP-Seq percent confirming: | 81.3187 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCTGGACTTGCATACC |
| Suggested primer 2: | AGGCAATATGGTGTGCAGCT |