| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040366 |
| Chromosome: | chromosome 8 |
| Location: | 3838049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381550 | (1 of 10) PTHR31867//PTHR31867:SF1 - FAMILY NOT NAMED // EXPANSIN-A1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATCAAACAGGAGTGCCAGCGCGGGGTTGTCGCGCGCGGGCGGGTTGG |
| Internal bar code: | TTAGTATTTGGGCGATTAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1421 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTACCCCGCCAACCAGTA |
| Suggested primer 2: | TTGCTACTGCCACATACGCA |