Insertion junction: CLIP2.040369_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.040369
Chromosome:chromosome 6
Location:5851485
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g289400 PDE11 (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAGCAGCCACATCAAGGAAGCGAACTACGACGTGCGTGAGGGCGCCGGC
Internal bar code:CGCAGCCGACTCTGTGGCGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2984
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:AGTACTGCGCACTCTGTCAC
Suggested primer 2:AGTCATCCGGGATCTGGTCA