Insertion junction: CLIP2.040383_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.040383
Chromosome:chromosome 10
Location:4230713
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449450 (1 of 41) PTHR10157 - DOPAMINE BETA HYDROXYLASE RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGACACACCTGACTCGCGCCGACCCGCCTTGCCCGCCACCTTCCGCCC
Internal bar code:CGTATGTCGGAGTGGTGGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTTCCTTACTTCCCTGCT
Suggested primer 2:TGCGGTCAACACGATACACA