| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.040414 |
| Chromosome: | chromosome 14 |
| Location: | 126306 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTCGCCGCCTGCGGCCCCGTGCTCGTCCTTCTCGTCCTCCCCCTCGT |
| Internal bar code: | GAAGGACAGTAACGGAAAAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACACGCACACACACGTA |
| Suggested primer 2: | GAGCACGGGTACTACATGGG |