Insertion junction: CLIP2.040418_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040418
Chromosome:chromosome 6
Location:2361913
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268100 HTB10 (1 of 27) K11252 - histone H2B (H2B); Histone H2B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATGCCGGTGTCGGGGTGCACCTGCTTGAGCACCTTGTAGATGTACAG
Internal bar code:AGACAGGACGTACTTCGAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:788
LEAP-Seq percent confirming:37.5
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCCCTTCTTCAGGTAGCG
Suggested primer 2:TGGTAAAGGCTGGCAGTGAG