| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040421 |
| Chromosome: | chromosome 12 |
| Location: | 2120165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAACGAAAAAGCCTTGCGGAGGGGATAGGGCCAACTAATGCGGACGGT |
| Internal bar code: | AGCGGGTGGGATAGGGGGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4054 |
| LEAP-Seq percent confirming: | 86.2069 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGAGCGACCTGTGAAAT |
| Suggested primer 2: | CTGTCAGTTTGTTGCTGGCC |