| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040425 |
| Chromosome: | chromosome 4 |
| Location: | 4070962 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233202 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTATCCTGGGGGAAGGCGGGGGTGGTCCTGTGCGGGTTCGGAAAGCAAG |
| Internal bar code: | CATTTGGATTTGTGCAAATCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCACTCATAGCCTGGCT |
| Suggested primer 2: | ATGGCGAAGAGGGAGAGAGT |