| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040426 |
| Chromosome: | chromosome 7 |
| Location: | 5962466 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g354550 | FAP65 | (1 of 18) 3.5.1.98 - Histone deacetylase / HDAC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGTGGCCAGCCGCCGCAGGCACAGTGCAAGCGTTCGAGAAGTCGCT |
| Internal bar code: | AGATATGTAAAGATTGAGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4254 |
| LEAP-Seq percent confirming: | 49.0566 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAGATAGGCTGGCATAGG |
| Suggested primer 2: | GATAGCGTGCTCGTGATGGA |