| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040430 |
| Chromosome: | chromosome 14 |
| Location: | 874774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613100 | (1 of 1) IPR008999 - Actin cross-linking | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATAATAATGTACGGCATGCCCGCACACACGCACCTCGCGCGCACACCG |
| Internal bar code: | CGTTACTGGATTGGCTATCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1728 |
| LEAP-Seq percent confirming: | 44.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTCACCAACAACGGCAC |
| Suggested primer 2: | CCGCAATCCGTCATTTCGTC |