Insertion junction: CLIP2.040443_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.040443
Chromosome:chromosome 14
Location:1254096
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616100 FKB11,FKB53 (1 of 1) K14826 - FK506-binding nuclear protein [EC:5.2.1.8] (FPR3_4); Peptidyl-prolyl cis-trans isomerase, FKBP-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCGTAGGCTTGGGAGACATGTACTGCCAGACCCATGCGCGACAAACAA
Internal bar code:AAGTGGGTTTAACCGCAAAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3382
LEAP-Seq percent confirming:94.186
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTAGCGTGTTAGGCCATA
Suggested primer 2:TCATCGTCGTCGTCGTCATC