| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040457 |
| Chromosome: | chromosome 3 |
| Location: | 2096420 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156400 | SMM8 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) 2.1.1.145 - Trans-aconitate 3-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAAGCAGACCCGCGCACACTCTTCCTAGACGCCGACCAGACCAGCCGC |
| Internal bar code: | CCACAACGGTGAACTTGCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 73.3333 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAACTACAGACCGCCCT |
| Suggested primer 2: | ATTTCCCGGAAATGGCCCAT |