Insertion junction: CLIP2.040458_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.040458
Chromosome:chromosome 6
Location:2852883
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272250 PHC27 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGAAGTCGGCAAAGTCCTTCATCACGGGGATGTACAGCCAGTTGGCG
Internal bar code:AGTTCAACGACGACGGATCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:359
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGCTGCATTGACGACCT
Suggested primer 2:CATGTCCTCGTGTTCCACCA