| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040464 |
| Chromosome: | chromosome 2 |
| Location: | 6644155 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389900 | HRP4 | Hydroxyproline-rich glycoprotein; (1 of 1) PTHR15744//PTHR15744:SF0 - BLOM7 // UPF0469 PROTEIN KIAA0907 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGCGCGCCTGGAGGCGCGTAGCCGTAGGGTGGGGCGTAGGACCCAG |
| Internal bar code: | AATTCCACCAAAATAGTGAGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3562 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACTGTGTACGCAGGGAAC |
| Suggested primer 2: | TACGCGGACACAATGTGACA |