Insertion junction: CLIP2.040486_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.040486
Chromosome:chromosome 9
Location:2684242
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801022 (1 of 12) IPR011010//IPR013762 - DNA breaking-rejoining enzyme, catalytic core // Integrase-like, catalytic domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGAACTGAAGCATGCTGGGGCTGGTGGCGCGAGGAACGGTGAAGGCCA
Internal bar code:GGGCGATCCATAGGGGGACTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2743
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGGAGATGACGGAGCAGTAC
Suggested primer 2:CCGTTTTATGCATCCGGCTG