Insertion junction: CLIP2.040498_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040498
Chromosome:chromosome 2
Location:5179422
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112000 AOT7 Amino acid transporter; (1 of 1) K14997 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 11 (SLC38A11) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGAACGCAAATCCCAGCACCGCAAACGCCTCCGGCAAGTGCTCATCCACC
Internal bar code:TACCGTGCGTCAAGTTTCTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3654
LEAP-Seq percent confirming:98.5075
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGCTCATCGCTGACCTTT
Suggested primer 2:TAGTGTGTGGTGACCATGCC