| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040507 |
| Chromosome: | chromosome 10 |
| Location: | 5232023 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456150 | HLD3 | (1 of 1) PTHR10992:SF701 - SERINE HYDROLASE-RELATED; Haloalkane dehalogenase-like hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGGCGCCCCGCCCGCCGCCGGCGACACCACCTCCCGGATGAACGCAG |
| Internal bar code: | GTGTAGAGCGGTACATAGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4216 |
| LEAP-Seq percent confirming: | 96.2406 |
| LEAP-Seq n confirming: | 128 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 133 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTTGCAGTGCACTGTG |
| Suggested primer 2: | AGGCAAAGTACATGACGCCA |