| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040523 |
| Chromosome: | chromosome 17 |
| Location: | 2593054 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716350 | GT90F32,GT90-32 | GT90 family protein 32; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTCATAATGACGGTTCATAAAGGACTATTGGTGAGCCGTTGCTCGGCG |
| Internal bar code: | ACAAAACTGTTTTGGCTATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1185 |
| LEAP-Seq percent confirming: | 4.91803 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 232 |
| LEAP-Seq n unique pos: | 244 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTAAGGCATTGTGCTCT |
| Suggested primer 2: | GCATGTGCATGTGTGTGTGT |