| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040524 |
| Chromosome: | chromosome 13 |
| Location: | 3649645 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588155 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCTTCCAAATGATACACGTATTCATGCAGGACGCTCTGAGCAAGGCG |
| Internal bar code: | CGTGGACATAAGCGGTCTACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1034 |
| LEAP-Seq percent confirming: | 47.3684 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATGGCGTCAAGGAGCAA |
| Suggested primer 2: | GCTTGCTCGAGCTGCATTAC |