| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040533 |
| Chromosome: | chromosome 7 |
| Location: | 4937329 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346800 | FAO8 | FAD-dependent oxidoreductase; (1 of 1) PTHR13847//PTHR13847:SF204 - FAD NAD BINDING OXIDOREDUCTASES // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCTCTTTAACACACACTCACGTCTGGGCGGGCTTTCGTTTCGTTCT |
| Internal bar code: | ACAGCGCGGTGGTCCGAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGTAGGGAGAGAGGGC |
| Suggested primer 2: | CGCCGTCTACATTTTGCTCG |