| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.040537 |
| Chromosome: | chromosome 17 |
| Location: | 2561149 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716050 | (1 of 1) PTHR20953:SF2 - PROTEIN SEEDLING PLASTID DEVELOPMENT 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCTTATGGGGACGTCGATGCCGCGGGGCAGCGGCATGGGGAGGGTG |
| Internal bar code: | GTTAGTAGTGGGATCTTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1909 |
| LEAP-Seq percent confirming: | 65.7895 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGATGGTGGCTGACTCG |
| Suggested primer 2: | CATGCAGGTGTGTGTGTGTG |