| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040538 |
| Chromosome: | chromosome 12 |
| Location: | 686105 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493000 | (1 of 1) IPR006153//IPR020846 - Cation/H+ exchanger // Major facilitator superfamily domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGCGGTGGTGGTGGGGTGGGGGGTTGGCAAGATGGCACAGAGAAT |
| Internal bar code: | ATGTTCAAGCATTTCAAGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGCTATCCTCTAACTCG |
| Suggested primer 2: | GAACGTGATCATCTTGCGCC |