Insertion junction: CLIP2.040547_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.040547
Chromosome:chromosome 5
Location:1088264
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g248000 CGL29,LESV1 Like early starvation 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACCCCACCCCACCCCAACCCAACCCGCGCCAATCCAACCCACCCCGC
Internal bar code:TTGTTTTGTACCCACGGGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:187
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCCGGAATAGGGAAGGGTA
Suggested primer 2:CCCGTCGCAAGGATTCTGAT