Insertion junction: CLIP2.040559_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.040559
Chromosome:chromosome 10
Location:6430349
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g464750 NAT25 (1 of 5) PTHR23091:SF255 - ACYL-COA N-ACYLTRANSFERASES-LIKE PROTEIN; N-acetyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTACTCCAATCCTGTGCGTCCCGTTGGTGCGCTTGGTTTGGTGGTGCG
Internal bar code:TGATTCCGCCTATTCTTGTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1255
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CACGAAGCAAGTGGTTGTGG
Suggested primer 2:GGCGAACACACAACCTGAAC