| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.040565 |
| Chromosome: | chromosome 1 |
| Location: | 6076973 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043000 | (1 of 1) PTHR30544//PTHR30544:SF6 - 23S RRNA METHYLTRANSFERASE // RADICAL SAM DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCGACGCTCGTCTTGGGAGCACAATTTGGTGCGCGGTCGGGCTTGTGG |
| Internal bar code: | ATCCCCCCCCTCTCACAAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1637 |
| LEAP-Seq percent confirming: | 80.3279 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGTAATCGCCGAGACA |
| Suggested primer 2: | GCACCATACCCTCATCGGAG |