| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040567 |
| Chromosome: | chromosome 6 |
| Location: | 7259023 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800731 | (1 of 17) PF00415 - Regulator of chromosome condensation (RCC1) repeat (RCC1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATCCCCCCAGCAATAGACTTCTTGAAGGCGTGTAGATACTGCATCAA |
| Internal bar code: | GAGCGTGTATATGCTGTCCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2611 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTATATTGCGGCAGCCAT |
| Suggested primer 2: | TCTCACGTCCAAGCTGACAC |