| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040575 |
| Chromosome: | chromosome 7 |
| Location: | 985187 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g319320 | ACD2 | 1-aminocyclopropane-1-carboxylate deaminase; (1 of 1) PTHR10314//PTHR10314:SF79 - SER/THR DEHYDRATASE, TRP SYNTHASE // DOF-TYPE TRANSCRIPTION FACTOR TMO6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGCTGCAGGTAGCTAGAGCTGACGCGGGCGAGGGCTGGGGCTGGAG |
| Internal bar code: | ACTTAGCTCCCGGACCACTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1091 |
| LEAP-Seq percent confirming: | 9.67742 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCCTCCAGTCCAATCG |
| Suggested primer 2: | GACAGGTGGGGACTAGGGAT |