| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040579 |
| Chromosome: | chromosome 6 |
| Location: | 8356127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307600 | (1 of 1) K11290 - template-activating factor I (SET, TAF1, I2PP2A); Nucleosome assembly protein | CDS | |
| Cre06.g307650 | (1 of 1) PF12138 - Spherulation-specific family 4 (Spherulin4) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGGCGAGGGCGAGGAGGCGGGCGGGCTGGAGGCCGGCGAGGGCGAG |
| Internal bar code: | TATATTGCGGACGGCTCGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3295 |
| LEAP-Seq percent confirming: | 63.1579 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCACTGCTCTGCCTGTA |
| Suggested primer 2: | TGAACAGCGCAACGTTGAAG |