Insertion junction: CLIP2.040583_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.040583
Chromosome:chromosome 3
Location:8144832
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g200750 DMC1 Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGATAGTGGACGACTCAGATGCGGCGAGTGGTGGGGAAGGCGAAAGCGA
Internal bar code:CCGTGTTAGTAGGGTCGAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:5077
LEAP-Seq percent confirming:96.7742
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGATCAGGTCTTCCTCCT
Suggested primer 2:GAGACATTCTGGGGGTGGTG