Insertion junction: CLIP2.040583_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.040583
Chromosome:chromosome 3
Location:8144855
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g200750 DMC1 Putative DNA methylase; (1 of 1) IPR027417//IPR029063 - P-loop containing nucleoside triphosphate hydrolase // S-adenosyl-L-methionine-dependent methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATCTGAGTCGTCCACTATCACGACATCGCTGTCCTCGTCGTCACCCTC
Internal bar code:CCGTGTTAGTAGGGTCGAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1265
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACATTCTGGGGGTGGTG
Suggested primer 2:GGGGATCAGGTCTTCCTCCT