| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.040595 |
| Chromosome: | chromosome 8 |
| Location: | 2242322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g370500 | (1 of 1) IPR013024//IPR017939 - Butirosin biosynthesis, BtrG-like // Gamma-glutamylcyclotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTGGTATGGTCCAGGGAGTAGTGACCGTGAATGCGCTACGGGAGTGCA |
| Internal bar code: | GAGACTCAGATTGGTAGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACACGAAACATTGGCGG |
| Suggested primer 2: | TCTTTGCACAGTCGGGATCC |