Insertion junction: CLIP2.040606_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.040606
Chromosome:chromosome 2
Location:5476590
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g114250 MEN2 Naphthoate synthase; (1 of 1) 4.1.3.36 - 1,4-dihydroxy-2-naphthoyl-CoA synthase / o-succinylbenzoyl-CoA 1,4-dihydroxy-2-naphthoate-lyase (cyclizing) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATCGGCCTTCCCATAGTGCTATCCCACACCCCACACGCCCCCGGCGCC
Internal bar code:CAGGGGTCAAAGTAAAAGTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:276
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGCGTACTTGGTTTCAGA
Suggested primer 2:TACTGGCGGGTATTGGAGGA